Boh Chang Lin, Alex Hogg


Thirty SPF pigs known to be free of Streptococcus suis infection were randomly selected from four litters at 10 to 12 days of age and allocated to 20 vaccinates and 10 controls. Each of the vaccinates was injected intramuscularly with a 1 ml dose of a S. suis bacterin at 10-12 days of age, followed by a 2.0 ml dose at 26 days of age. All of the animals were challenged with S. suis 16 days following the second vaccination. The vaccinates were protected and the controls showed clinical signs and necropsy lesions. Western blot analysis identified the special proteins between 35 and 50 kD that were recognized by all sera from pigs vaccinated twice and also by sera from pigs surviving challenge. These 35-50 kD proteins were not recognized by sera from prevaccinates and negative controls. An indirect ELISA using 35-50 kD proteins for coating plates was standardized for the detection of specific antibodies following vaccination and challenge.

Key words: Streptococcus suis, immunoglobulin G

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